Principle of the ELISA Method in the Pratham Kit

The Pratham HBsAg ELISA Test Kit utilizes a sandwich ELISA principle which is the most preferable in catching viral antigens.

Overview of the Workflow :

1. Antibody-Coated Wells :
   Specific anti-HBsAg antibodies are used to pre-coat the microplate wells.
2. Sample Incubation :
   Test samples are added, allowing the coated antibodies to bind HBsAg, if it is there.
3. Enzyme Conjugate Reaction :
   The antigen-antibody complex is formed when a secondary antibody that is linked to the enzyme binds to HBsAg.
4. Substrate Addition :
   Then, a chromogenic substrate (usually TMB) is added which produces a color change that is measurable.
5. Absorbance Measurement :
   The reaction is read at the specified wavelength with the ELISA reader.

This method guarantees signal clarity that is very high, background noise that is very minimal, and very good discrimination between positive and negative samples.

Why Hospitals & Labs Prefer the Pratham HBsAg ELISA Kit

1. High Analytical Accuracy

Pratham ELISA kit, with its declared 100% sensitivity and specificity, allows making decisions confidently in high-risk areas such as surgical units, blood banks, and dialysis centers.

2. Appropriate for Medium to High Throughput Labs

The 96-test format fits perfectly for hospitals and diagnostic labs that are processing batch samples on a daily or weekly basis.

3. Standardized & Friendly to Users Protocol

The assay uses well-known ELISA steps and thus guarantees easy integration into the workflow and minimal training needed for the staff.

4. Laboratory-Compatible Configuration

• Compatible with the most common ELISA readers and washers.
• Reagents are stable when kept at 2–8°C thus easy storage.
• Color-coded parts or clearly labeled components make it easier to use.

5. Reliable Brand Value – Pratham

The Pratham is a brand that intentionally wants to be seen as a very strong diagnostic brand highlighting quality, precision, and reliability of the test kits in all applications. Pratham label is the guarantee of uniform performance in hospitals and laboratories.

Applications in Clinical & Diagnostic Settings

• Routine Patient Screening

Enables early detection of both acute and chronic HBV infections in persons showing symptoms as well as in those without symptoms.

• Blood Donor Screening

It is a must for the safety of the donor’s blood and avoidance of transfusion-transmitted infections.

• Preoperative Screening

It is a prerequisite for surgical interventions to ensure safe dealing and minimizing cross-contamination.

• Maternal Health Programs

It is a help in detecting the mothers that are infected and in minimizing the infant’s exposure during delivery.

• Occupational Health Testing

It is a measure of safety for the health care staff and involves screening for high-risk exposures.

• Public Health Surveillance

It is one of the applications in mass screening programs and epidemiological studies owing to its ability to process high volumes of samples.

Best Practices When Using Pratham HBsAg ELISA Kits

Use the following measures to get the best and most accurate results :

• Sample collection guidelines should be strictly followed—hemolysis and improper storage must be avoided.
• The laboratory instruction manual should provide the correct incubation times and temperatures.
• Positive and negative controls must be run with each plate.
• Calibrated pipettes should be used, and good laboratory practices should be maintained.
• For traceability, record batch numbers and results.
• Biohazardous materials should be disposed of in a safe way.

By following these procedures, accuracy will be enhanced and standards for diagnostic accreditation will be reached.